Evidence for a differential translocation of actinides across human lung epithelial cell monolayer in vitro according to their physicochemical properties and the presence of a chelating agent

The epithelial cell plays a key role in the transfer of radionuclides from lungs to blood following pulmonary exposure. present study was designed evaluate across human lung cells various actinides (plutonium, americium and uranium), influence physicochemical properties plutonium compounds chelating agent diethylene triamine pentaacetic acid (DTPA). To address this question, Calu-3 grown bicameral culture system were used. integrity barrier evaluated by measuring transepithelial electrical resistance (TEER) passage fluorescent marker, lucifer yellow. Activity measurement basal compartment periodic collection medium made 2 h seven days. facilitate data handling analysis, statistical tool STATBIODIS results indicate differences for different elements, according Pu properties. Though extents, DTPA always increased Am cells, without altering barrier. This vitro model, mimicking translocation blood, can represent valuable further understand underlying mechanisms controlling process.